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J Immunoassay Immunochem 2017;38(4):355-77

A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays.

Tam JO, de Puig H, Yen CW, Bosch I, Gomez-Marquez J, Clavet C, Hamad-Schifferli K, Gehrke L

Abstract

Point-of-care (POC) diagnostics such as lateral flow and dipstick immunoassays use gold nanoparticle (NP)-antibody conjugates for visual readout. We investigated the effects of NP conjugation, surface chemistries, and antibody immobilization methods on dipstick performance. We compared orientational, covalent conjugation, electrostatic adsorption, and a commercial conjugation kit for dipstick assays to detect dengue virus NS1 protein. Assay performance depended significantly on their conjugate properties. We also tested arrangements of multiple test lines within strips. Results show that orientational, covalent conjugation with PEG shield could improve NS1 detection. These approaches can be used to optimize immunochromatographic detection for a range of biomarkers.

Category: Journal Article
PubMed ID: #27982728 DOI: 10.1080/15321819.2016.1269338
Includes FDA Authors from Scientific Area(s): Regulatory Affairs
Entry Created: 2016-12-20 Entry Last Modified: 2017-10-01
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