Scientific Publications by FDA Staff

J Clin Microbiol 2004 Apr;42(4):1723-1726

Genotyping of Enteric Adenoviruses by Using Single-Stranded Conformation Polymorphism Analysis and Heteroduplex Mobility Assay.

Soares CC, Volotao EM, Albuquerque MC, Nozawa CM, Linhares RE, Volokhov D, Chizhikov V, Lu X, Erdman D, Santos N

Santos N, Univ Fed Rio de Janeiro, Dept Virol, Inst Microbiol, Cidade Univ,CCS,Bl I, BR-21941590 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro, Dept Virol, Inst Microbiol, BR-21941590 Rio De Janeiro, Brazil Univ Estadual Londrina, Dept Microbiol, BR-86061970 Londrina, PR, Brazil US FDA, Ctr Biol Evaluat & Res, Rockville, MD 20852 USA Ctr Dis Control & Prevent, Div Viral & Rickettsial Dis, Atlanta, GA 30333 USA

Single-stranded conformation polymorphism (SSCP) analysis and heteroduplex mobility assays (HMAs) were used to identify and genotype enteric adenoviruses (EAd). The results were compared to those of restriction endonuclease assays, species-specific PCRs, and direct nucleotide sequence analyses. Of the 31 stool samples tested, 15 isolates were identified as EAd and 7 were identified as nonenteric Ad by all methods. An agreement of 100% was found between the SSCP and HMA results.