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U.S. Department of Health and Human Services

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Title:  Anesthetic effect of eugenol and menthol on handling stress in Macrobrachium rosenbergii.
 
Authors:  Saydmohammed M, Pal AK
 
Drug:  Eugenol+Menthol
 
Fish species:  Shrimp, giant freshwater prawn
 
Date:  2009
 
Sample:  muscle
 
Ave Weight (gm):  32
 
Ave Water temp (°C):  30
 
Water:  Freshwater
 
Dosage:  0.2 mL/L Eugenol + 0.2 mL/L Menthol 30 min
 
Route:  BATH
 
Comments:  Eugenol showed similar anesthetic property to that of a mixture of eugenol and menthol, but menthol used alone was ineffective. The minimum dose to induce anesthesia within 30 min was 200 uL/L. Induction time=29.1 min. Recovery time=18.5 min. Oxygen consumption=2 mg O2 kg/h. Eugenol accumulated in highest amount (35.2 mg/kg) immediately after anesthetic mixture treatment. Eugenol level reduced to 12 mg/kg after 12h. Eugenol was below detectable limits following 24 and 48h. Menthol was detected at 3 mg/kg immediately after anesthetic mixture treatment. Methol was below detectable limits after 12, 24, and 48h. Results indicate that eugenol and menthol residues accumulate in the tissues, however a 24 hour withdrawl period clears the residues from the tissue.
 
Citation:  Aquaculture 298:162-167
 
Link:  http://dx.doi.org/10.1016/j.aquaculture.2009.10.020  Opens a new window
 
Genus Species:  Macrobrachium rosenbergii
 
Drug Class:  Anesthetic-Methoxyphenol & Organic-Menthol
 
Wt gm-detail:  32+/-2.1
 
Water temp (°C)-detail:  30
 
Dosage-detail:  200 ul/L Eugenol +200 ul/L Menthol
 
Method:  GC
 
Authors-red:  Saydmohammed M, Pal AK
 
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