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U.S. Department of Health and Human Services

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Title:  Comparative efficacy of MS-222 and benzocaine as anaesthetics under simulated transport conditions of a tropical ornamental fish Puntius filamentosus (Valenciennes).
 
Authors:  Pramod PK, Ramachandran A, Sejeevan TP, Thampy S, Pai SS
 
Drug:  MS-222
 
Fish species:  Barb, blackspot
 
Date:  2010
 
Sample:  blood, plasma
 
Ave Weight (gm):  12
 
Ave Water temp (°C):  22
 
Water:  Freshwater
 
Dosage:  40 mg/L 48h
 
Route:  BATH
 
Comments:  Fish were kept for 48h in water at 22C and after sampling, remaining fish were transfered to tanks with water at 28C. Fish euthanized with lethal dose concentration of 200 mg/L of MS-222 before sampling.The lowest increase in plasma cortisol levels was in MS-222 treated group followed by benzocain treatment within the first 6h of packing. Increase significantly lower (P<0.05) than nonsedated fish however no significant difference between MS-222 and benzocaine. Increase in blood glucose was significantly lower than nonsedated fish but not significantly different than the benzocaine treatment. Post transport mortality after 7d was 18% compared to 43% in nonsedated fish.
 
Citation:  Aquaculture Research 41:309-314
 
Link:  http://dx.doi.org/10.1111/j.1365-2109.2009.02333.x  Opens a new window
 
Genus Species:  Puntius filamentosus
 
Drug Class:  Anesthetic-Tricaine
 
Wt gm-detail:  12+/- 1g
 
Water temp (°C)-detail:  48 h:22+/-1C, 7d:28+/-1C
 
Dosage-detail:  40 mg/L
 
Method:  ELISA
 
Authors-red:  Pramod PK, Ramachandran A, Sejeevan TP, Thampy S, Pai SS
 
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