An internal biomérieux investigation was performed.The investigation was initiated due to false susceptible vancomycin (va) on vitek® 2 (v7.01) ast-p586 cards involving an enterococcus faecium strain.The reference method (broth microdilution, bmd), which was the method used for vancomycin development (formulation va04n) on the vitek 2 ast-p586 card, gave: bmd va mic > 64 mg/l r.On the vitek 2 v7.01 ast-p586 card (aes parameters : eucast + phenotypic), four (4) ast-p586 cards (one from the customer lot n°1, cl1 3660564203, one from the customer lot n°2, cl2 3660477403 one from the customer lot n°3, cl3 3660600403 and one from a random lot rl 3660410203) were tested.Cl1/cl2/cl3: va mic = 8 mg/l r with "van b like" phenotype.Rl: va mic = 0.5 mg/l s with "wild" phenotype.The mic values on cl1, 2 and 3 are in essential agreement compared to the reference result.The false susceptible vancomycin on vitek 2 is reproduced on the rl (= 0.5 mg/l).The underestimation of vancomycin on the vitek 2 ast-p586 card was confirmed in-house.This underestimation leads to a non-detection of the van b like phenotype on the rl only.A trend analysis was completed for this device issue, resulting in no implication of a trend.
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