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Dev Biol 2006 Feb 15;290(2):482-94

Transcriptional activation by extradenticle in the Drosophila visceral mesoderm.

Stultz BG, Jackson DG, Mortin MA, Yang X, Beachy PA, Hursh DA

Hursh DA, US FDA, Cellular & Tissue Therapy Branch, Ctr Biol Evaluat & Res, HFM-730,Bldg 29B,Rm 1E16,8800 Rockville Pike, Bethesda, MD 20892 USA US FDA, Cellular & Tissue Therapy Branch, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA NICHHD, Biochem Lab, Natl Canc Inst, NIH, Bethesda, MD 20892 USA NICHHD, Biochem Lab, Genet Mol Lab, NIH, Bethesda, MD 20892 USA NCI, Biochem Lab, NIH, Bethesda, MD 20892 USA


Decapentaplegic (dpp) is a direct target of Ultrabithorax (Ubx) in parasegment 7 (PS7) of the embryonic visceral mesoderm. We demonstrate that extradenticle (exd) and homothorax (hth) are also required for dpp expression in this location, as well as in PS3, at the site of the developing gastric caecae. A 420 bp element from dpp contains EXD binding sites necessary for expressing a reporter gene in both these locations. Using a specificity swap, we demonstrate that EXD directly activates this element in vivo. Activation does not require Ubx, demonstrating that EXD can activate transcription independently of homeotic proteins. Restoration is restricted to the domains of endogenous dpp expression, despite ubiquitous expression of altered specificity EXD. We demonstrate that nuclear EXD is more extensively phosphorylated than the cytoplasmic form, suggesting that EXD is a target of signal transduction by protein kinases.

Category: Journal Article
PubMed ID: #16403493
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2011-10-04 Entry Last Modified: 2012-08-29