Scientific Publications by FDA Staff

J Recept Signal Transduct Res 2009;29(3-4):202-10

Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system-Epic system.

Wu M, Long S, Frutos AG, Eichelberger M, Li M, Fang Y

The Epic system, a high-throughput label-free optical biosensor system, is applied for the biochemical interrogation of phosphor-specific interactions of the 14-3-3 protein and its substrates. It has shown the capability not only for high-throughput characterization of binding rank and affinity but also for the exploration of potential interacting kinases for the substrates. A perspective of biochemical applications for diagnostics and biomarker discovery, as well as cell-based applications for endogenous receptors and viral infection characterization, are also provided.