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U.S. Department of Health and Human Services

Scientific Publications by FDA Staff

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Photochem Photobiol 1994 Sep;60(3):225-30

Photoactivated gilvocarcin V induces DNA-protein crosslinking in genes for human ribosomal RNA and dihydrofolate reductase.

Matsumoto A, Fujiwara Y, Elespuru RK, Hanawalt PC


The nature of DNA interactions with photoactivated gilvocarcin V has been analyzed at the gene level in both rRNA and dihydrofolate reductase genes of human fibroblasts, utilizing a modified Southern hybridization technique. Neither interstrand DNA crosslinking nor RNA linkage to DNA was detected. However, we consistently observed in both genes retarded DNA bands appearing in a dose-dependent fashion following exposure to photoactivated gilvocarcin V. These retarded bands were enhanced when genomic DNA was prepared without proteinase K treatment, suggesting involvement of protein in this DNA interaction. Because these bands disappear following proteinase K treatment, it is probable that photoactivated gilvocarcin V induces DNA-protein crosslinking.

Category: Journal Article
PubMed ID: #7972373 DOI: 10.1111/j.1751-1097.1994.tb05095.x
Includes FDA Authors from Scientific Area(s): Medical Devices
Entry Created: 2012-11-13