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Photochem Photobiol 1977 May;25(5):477-82

Radiation enhanced reactivation of nuclear replicating mammalian viruses.

Bockstahler LE, Lytle CD


When CV-1 monkey kidney cells were UV-irradiated (0¿18 J/m2) or X-irradiated (0¿10krads) before infection with UV-irradiated simian adenovirus 7 (SA7) or simian virus 40 (SV40), increases in the infectivity of these nuclear replicating viruses as measured by plaque formation were observed. These radiation enhanced reactivations, UV enhanced reactivation (UVER) and X-ray enhanced reactivation (X-ray ER), occurred both when virus infection immediately followed irradiation of the cells (except for X-ray ER with SA7) and when virus infection was delayed until 3¿5 days after cell irradiation. While there was little difference in the levels of reactivation of UV-irradiated SV40 between immediate and delayed infection, delayed infection resulted in higher levels of reactivation of SA7. X-ray enhanced reactivation of UV-irradiated Herpes simplex virus persisted for several days but did not increase. Thus, X-ray enhanced and UV enhanced reactivations of these mammalian viruses were relatively long-lived effects. Essentially no UVER or X-ray ER was found in CV-1 cells for either immediate or delayed infection with UV-irradiated vaccinia virus or poliovirus, both of which replicate in the cell cytoplasm. These results suggest UVER and X-ray ER in mammalian cells may be restricted to viruses which are replicated in the cell nucleus.

Category: Journal Article
PubMed ID: #197551 DOI: 10.1111/j.1751-1097.1977.tb09173.x
Includes FDA Authors from Scientific Area(s): Medical Devices
Entry Created: 2012-11-30