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U.S. Department of Health and Human Services

Scientific Publications by FDA Staff

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Arthritis Rheum 1997 Apr;40(4):694-703

Induction of alternative splicing of HLA-B27 by bacterial invasion.

Huang F, Yamaguchi A, Tsuchiya N, Ikawa T, Tamura N, Virtala MM, Granfors K, Yasaei P, Yu DT


OBJECTIVE: Alternative splicing of certain class I major histocompatibility complex pre-messenger RNA (pre-mRNA) is known to lead to generation of a cell-free soluble protein analog. This study was undertaken to examine whether this process occurs with HLA-B27, whether the process is modified by arthritis-causing bacteria, and whether the assembly of the soluble molecules follows the same pathway as the integral parent molecules. METHODS: Alternative splicing of pre-mRNA was analyzed by reverse transcriptase-polymerase chain reaction, and assembly of soluble HLA-B27 by immunoprecipitation followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. RESULTS: There was alternative splicing of the pre-mRNA of HLA-B27. The process could be amplified by invasion with Salmonella or Yersinia bacteria. The soluble HLA-B27 was assembled in a pathway similar to that of the parent molecule. CONCLUSION: The association between arthritis-causing bacteria and HLA-B27 positive cells is a complex event. Soluble HLA-B27 is a potential key player.

Category: Journal Article
PubMed ID: #9125251 DOI: 10.1002/art.1780400414
Includes FDA Authors from Scientific Area(s): Food
Entry Created: 2012-12-26