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U.S. Department of Health and Human Services

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Vaccine 2013 Aug 20;31(37):3987-94

Development of a simple, rapid, sensitive, high-throughput luciferase reporter based microneutralization test for measurement of virus neutralizing antibodies following respiratory syncytial virus vaccination and infection.

Fuentes S, Crim RL, Beeler J, Teng MN, Golding H, Khurana S


We have established a new reporter gene-based RSV neutralization test using Renilla luciferase. The RSV-luciferase neutralization test (RSV-Luc-NeuT) is a simple, rapid, high throughput, and less labor intensive functional serological assay than the traditional RSV-PRNT, capable of measuring a broad range of anti-RSV neutralizing antibodies targeting both RSV-F and RSV-G proteins. Specificity and sensitivity of the RSV-Luc-NeuT are comparable to the RSV-PRNT. Panels of pre-vaccination and post-vaccination animal sera, monoclonal antibodies and animal polyclonal anti-RSV sera confirmed assay specificity. A panel of 60 human sera demonstrated high assay sensitivity for measurement of RSV neutralizing antibodies that strongly correlated with the RSV-PRNT titers (R2=0.864). Neutralization in the presence of guinea pig complement (GPC) increased PRNT titers more than the RSV-Luc-NeuT neutralizing antibody titers for these human sera. This newly developed simple, high throughput, RSV-Luc-NeuT could be easily automated and applied in measurement of RSV neutralization titers in large vaccine trials.

Category: Journal Article
PubMed ID: #23742994 DOI: 10.1016/j.vaccine.2013.05.088
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2013-03-21 Entry Last Modified: 2013-10-20