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U.S. Department of Health and Human Services

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J Food Prot 2013 May;76(5):867-70

Multilaboratory Validation of a Luminex Microbead-Based Suspension Array for the Identification of the 11 Most Clinically Relevant Shiga Toxin-Producing Escherichia coli O Serogroups.

Lin A, Kase JA, Moore MM, Son I, Tran N, Clotilde LM, Jarvis K, Jones K, Kasturi K, Nabe K, Nucci M, Wagley GS, Wang F, Ge B, Hammack TS


Rapid and high-throughput identification and serotyping of Shiga toxin-producing Escherichia coli (STEC) O serogroups is important for detecting, investigating, and controlling STEC infection outbreaks and removing hazardous products from commerce. A Luminex microbead-based suspension array has been developed to identify the 11 most clinically relevant STEC serogroups: O26, O45, O91, O103, O104, O111, O113, O121, O128, O145, and O157. Here we present results of a blinded multilaboratory collaborative study involving 10 participants from nine laboratories using 55 unknown strains. From the total 495 analyses, two false-positive and three false-negative results were obtained, indicating the assay to be a rapid, high-throughput, and robust method for identifying clinically relevant STEC serogroups.

Category: Journal Article
PubMed ID: #23643130 DOI: 10.4315/0362-028X.JFP-12-468
Includes FDA Authors from Scientific Area(s): Regulatory Affairs Food Animal and Veterinary
Entry Created: 2013-05-08 Entry Last Modified: 2013-11-24