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Emerg Infect Dis 2015 Mar;21(3):400-8

Nanomicroarray and multiplex next-generation sequencing for simultaneous identification and characterization of influenza viruses.

Zhao J, Ragupathy V, Liu J, Wang X, Vemula SV, El Mubarak HS, Ye Z, Landry ML, Hewlett I

Abstract

Conventional methods for detection and discrimination of influenza viruses are time consuming and labor intensive. We developed a diagnostic platform for simultaneous identification and characterization of influenza viruses that uses a combination of nanomicroarray for screening and multiplex next-generation sequencing (NGS) assays for laboratory confirmation. The nanomicroarray was developed to target hemagglutinin, neuraminidase, and matrix genes to identify influenza A and B viruses. PCR amplicons synthesized by using an adapted universal primer for all 8 gene segments of 9 influenza A subtypes were detected in the nanomicroarray and confirmed by the NGS assays. This platform can simultaneously detect and differentiate multiple influenza A subtypes in a single sample. Use of these methods as part of a new diagnostic algorithm for detection and confirmation of influenza infections may provide ongoing public health benefits by assisting with future epidemiologic studies and improving preparedness for potential influenza pandemics.


Category: Journal Article
PubMed ID: #25694248 DOI: 10.3201/eid2103.141169
PubMed Central ID: #PMC4344273
Includes FDA Authors from Scientific Area(s): Biologics Medical Devices
Entry Created: 2016-02-29
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