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J Gen Virol 2014 Dec 12 [Epub ahead of print]

Chimeric NA and mutant PB1 gene constellation improves growth and yield of H5N1 vaccine candidate virus.

Plant EP, Ye Z

Abstract

We previously showed that a mutated PB1 gene improved the growth kinetics of a H3N2 influenza reassortant. Here we show that the same mutations improve the growth kinetics of a virus containing the A/VietNam/1203/2004 (H5N1) HA and NA. Total protein yield and neuraminidase activity were increased when a chimeric NA was included. These increases indicate that the synergistic effect is due to the gene constellation containing both the altered PB1 gene and the chimeric NA gene.


Category: Journal Article
PubMed ID: #25502649 DOI: 10.1099/jgv.0.000025
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2014-09-24 Entry Last Modified: 2014-12-17
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