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Sci Rep 2017 Aug 2;7(1):7149

Femtogram level sensitivity achieved by surface engineered silica nanoparticles in the early detection of HIV infection.

Chunduri LAA, Kurdekar A, Haleyurgirisetty MK, Bulagonda EP, Kamisetti V, Hewlett IK


We have engineered streptavidin labelled Europium doped fluorescent silica nanoparticles which significantly increased sensitivity without compromising the specificity of the immunoassay. As a proof of concept, a time resolved fluorescence based sandwich immunoassay was developed to detect HIV-1 p24 antigen in clinical specimens. The detection range of the silica nanoparticle based immunoassay (SNIA) was found to be between 0.02 to 500 pg/mL in a linear dose dependent manner. SNIA offers 1000 fold enhancement over conventional colorimetric ELISA. Testing of plasma samples that were HIV negative showed no false positive results in the detection of HIV-1 p24 antigen. This highly sensitive p24 assay can help improve blood safety by reducing the antibody negative window period in blood donors in resource limited settings where nucleic acid testing is not practical or feasible. This technology can also be easily transferred to a lab-on-a-chip platform for use in resource limited settings and can also be easily adopted for the detection of other antigens.

Category: Journal Article
PubMed ID: #28769052 DOI: 10.1038/s41598-017-07299-1
PubMed Central ID: #PMC5540927
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2017-08-13 Entry Last Modified: 2017-08-27