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AIDS Res Hum Retroviruses 2019 Apr;35(4):396-401

Cross-subtype detection of HIV-1 capsid p24 antigen using a sensitive europium nanoparticle assay.

Haleyur Giri Setty MK, Kurdekar A, Mahtani P, Liu J, Hewlett I


BACKGROUND: Accurate and early detection of diverse HIV-1 subtypes using currently available assays has been a major challenge. We report development of a sensitive Time Resolved Fluorescence (TRF) Europium Nanoparticle Immuno Assay (ENIA) for cross subtype detection of p24 antigen using broadly cross reactive antibodies. METHODS: Several antibodies were tested for optimal reactivity with antigens of diverse HIV-1 subtypes and CRFs. We tested HIV strains using this assay for sensitivity and quantification ability at the pico-gram (pg)/ml level. RESULTS: We identified two broadly cross reactive antibodies C65690M and ANT-152, which detected all strains of HIV tested. These two antibodies also yield a better signal to cut off ratios for the same amount of antigen tested in comparison to a commercial assay. CONCLUSION: Using a combination of appropriate C65690M and ANT-152 p24 antibodies capable of detecting all HIV types and highly sensitive time resolved fluorescence (TRF) based Europium Nano particle assay platform, we developed a sensitive p24 antigen assay that can detect HIV infection of all HIV subtypes and may be useful in early detection.

Category: Journal Article
PubMed ID: #30411969 DOI: 10.1089/AID.2018.0163
PubMed Central ID: #PMC6461306
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2018-02-25 Entry Last Modified: 2019-04-21