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Methods Mol Biol 2019;1911:421-32

Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA.

Major M, Law M

Abstract

Enzyme-linked immunosorbent assays (ELISAs) enable rapid detection and quantitation of antibodies in samples. Such assays can be highly sensitive and can be performed in most laboratories with basic equipment. Although detecting binding antibodies to the surface proteins of most pathogens by ELISA is not always indicative of antibody function, i.e., neutralizing activity of antibodies, the results can be used as a first step toward more in-depth analysis of antibody responses. Here we describe a method that can be used to standardize ELISAs for the detection of HCV envelope antibodies across laboratories and provide adaptations of the method to further characterize antibody responses in serum samples.


Category: Journal Article
PubMed ID: #30593642 DOI: 10.1007/978-1-4939-8976-8_28
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2018-10-07 Entry Last Modified: 2019-01-26
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