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Sci Adv 2019 Jun 5;5(6):eaaw7396

Modular, tissue-specific, and biodegradable hydrogel cross-linkers for tissue engineering.

Guo JL, Kim YS, Xie VY, Smith BT, Watson E, Lam J, Pearce HA, Engel PS, Mikos AG


Synthetic hydrogels are investigated extensively in tissue engineering for their tunable physicochemical properties but are bioinert and lack the tissue-specific cues to produce appropriate biological responses. To introduce tissue-specific biochemical cues to these hydrogels, we have developed a modular hydrogel cross-linker, poly(glycolic acid)-poly(ethylene glycol)-poly(glycolic acid)-di(but-2-yne-1,4-dithiol) (PdBT), that can be functionalized with small peptide-based cues and large macromolecular cues simply by mixing PdBT in water with the appropriate biomolecules at room temperature. Cartilage- and bone-specific PdBT macromers were generated by functionalization with a cartilage-associated hydrophobic N-cadherin peptide, a hydrophilic bone morphogenetic protein peptide, and a cartilage-derived glycosaminoglycan, chondroitin sulfate. These biofunctionalized PdBT macromers can spontaneously cross-link polymers such as poly(N-isopropylacrylamide) to produce rapidly cross-linking, highly swollen, cytocompatible, and hydrolytically degradable hydrogels suitable for mesenchymal stem cell encapsulation. These favorable properties, combined with PdBT's modular design and ease of functionalization, establish strong potential for its usage in tissue engineering applications.

Category: Journal Article
PubMed ID: #31183408 DOI: 10.1126/sciadv.aaw7396
PubMed Central ID: #PMC6551165
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2019-06-16 Entry Last Modified: 2019-07-21