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U.S. Department of Health and Human Services

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Virology 2019 Oct;536:125-33

The Spodoptera frugiperda Sf9 cell line is a heterogeneous population of rhabdovirus-infected and virus-negative cells: Isolation and characterization of cell clones containing rhabdovirus X-gene variants and virus-negative cell clones.

Ma H, Nandakumar S, Bae EH, Chin PJ, Khan AS


The Sf9 cell line is broadly used for manufacturing baculovirus-expressed viral vaccines. We previously reported the presence of a novel, rhabdovirus in the Sf9 cell line, which contained a unique X gene (Sf-rhabdovirus; designated as X(+) in this paper). These results were extended by other reports describing an Sf-rhabdovirus variant in Sf9 cells, which lacked 320 nucleotides encompassing the X-gene and adjacent intergenic region (designated as X(-) in this paper), and the development of an Sf-rhabdovirus negative cell line. Here, we report that the Sf9 cell line is a mixed-cell population, based upon isolation of cell clones with distinct phenotypes: Sf-rhabdovirus-negative, X(+), and X(-). We also show that Sf-rhabdovirus X(+) and X(-) variants replicate independently in Sf-rhabdovirus-negative cells. These results shed light on the detection of different rhabdovirus variants by different laboratories using Sf9-derived cell clones and confirm that both X(+) and X(-) viruses are infectious in rhabdovirus-negative Sf9 cells.

Category: Journal Article
PubMed ID: #31494355 DOI: 10.1016/j.virol.2019.08.001
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2019-09-15 Entry Last Modified: 2019-09-29