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Title:
Comparative efficacy of MS-222 and benzocaine as anaesthetics under simulated transport conditions of a tropical ornamental fish Puntius filamentosus (Valenciennes).
Authors:
Pramod PK, Ramachandran A, Sejeevan TP, Thampy S, Pai SS
Drug:
Benzocaine
Fish species:
Barb, blackspot
Date:
2010
Sample:
blood, plasma
Ave Weight (gm):
12
Ave Water temp (°C):
22
Water:
Freshwater
Dosage:
20 mg/L 48h
Route:
BATH
Comments:
Fish were kept for 48h in water at 22 C and after sampling, remaining fish were transfered to tanks with water at 28 C.Fish euthanized with lethal dose concentration of 200 mg/L of MS-222 before sampling. The lowest increase in plasma cortisol levels was in MS-222 treated group followed by benzocain treatment within the first 6h of packing. Increase significantly lower (P<0.05) than nonsedated fish however no significant difference between MS-222 and benzocaine. Increase in blood glucose was significantly lower than nonsedated fish but not significantly different than the MS-222 treatment. Post transport mortality after 7d was 20% compared to 43% in nonsedated fish.
Citation:
Aquaculture Research 41:309-314
Link:
http://dx.doi.org/10.1111/j.1365-2109.2009.02333.x
Genus Species:
Puntius filamentosus
Drug Class:
Anesthetic-Benzocaine
Wt gm-detail:
12+/- 1g
Water temp (°C)-detail:
48 h:22+/-1C, 7d:28+/-1C
Dosage-detail:
20 mg/L
Method:
ELISA
Authors-red:
Pramod PK, Ramachandran A, Sejeevan TP, Thampy S, Pai SS
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