The genomic dna sample was sent to grifols ih center for next generation.Sequencing interrogated fy proximal promoter, exons 1-2 and the variant fy:c.299c>a were identified.This unreported variant was associated by cloning and sanger sequencing to fy*b allele (fy*b(299a) allele).Id core xt reported a predicted fyb+ positive phenotype, but fy:c.299c>a variant, not interrogated by id core xt, is associated with fyb- phenotype based on the serology result.This false positive result obtained by id core xt are considered a discrepant result and then a malfunction.This limitation is covered by the general assay limitations described in the id core xt package insert (limitation 1).
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