The genomic dna sample was sent to (b)(4) for bi-directional sequencing.Sequencing interrogated rh gene exons 1 to 10 and rh gen intron 2 and the following allele genotype was identified rhce*ce(ivs2+3139g),rhce*ce.The presence of unpublished variant rhce*c.Ivs2+3139a>g prevents binding of one of the id core xt rhce intron 2 probes which detect c antigen (rhce*c-specific109-bp insert) and consequently the prediction of c positive antigen.Id core xt reported a predicted c- phenotype, due to the presence of rhce*c.Ivs2+3139a>g variant which causes an allele drop-out, being the correct predicted phenotype c positive.This false negative result obtained by id core xt is considered a discrepant result and then a malfunction.This case report is associated with a new limitation of id core xt assay.
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