The genomic dna sample was sent to (b)(4) for bi-directional sequencing.Sequencing interrogated jk gene exons 3 to 10 and the following allele genotype was identified jka*(588a), jk*b(832c).Variant jk*c.588g>a is a published silent variant, p.196 pro/pro (erythrogene database.2016 blood advances vol.1 p.240).Variant jk*c.832t>c has not been previously reported.The presence of unpublished jk*c.832t>c variant in id core xt probes which detect jkb antigen prevents hybridization and consequently the prediction of jkb positive antigen.Id core xt reported a predicted jkb negative phenotype due to the presence of jk*c.832t>c variant which causes an allele-drop out, being the phenotype jkb positive.This false negative result obtained by id core xt is considered a discrepant result and then a malfunction.This case report is associated with a new limitation of id core xt assay.
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