Scientific Publications by FDA Staff

Vox Sang 1974 May;26(5):457-61

Dissociation of human albumin dimer by heating.

Finlayson JS, Reamer BL, Young AM

Exclusion chromatography was used to prepare human albumin dimer from normal serum albumin (human) which had been heated repeatedly at 56 °C and from un-heated fraction V which had been treated with ethanol and dried at 25 °C. When these purified dimer preparations were heated in solution for 10 h at 60°C in the presence of acetyltryptophan and sodium caprylate, 20¿23% of the protein was converted to monomer.