• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services

Scientific Publications by FDA Staff

  • Print
  • Share
  • E-mail

Search Publications



Starting Date

Ending Date

Order by

Entry Details

J Sep Sci 2015 Sep;38(17):2983-8

Determination of methylisothiazolinone and methylchloroisothiazolinone in cosmetic products by ultra high performance liquid chromatography with tandem mass spectrometry.

Wittenberg JB, Canas BJ, Zhou W, Wang PG, Rua D, Krynitsky AJ


Isothiazolinone biocides are broad-spectrum preservatives that are widely used in cosmetics, household and industrial products. An increase in the number of cases of allergic contact dermatitis to isothiazolinone preservatives, namely, methylisothiazolinone and methylchloroisothiazolinone, have been recently noticed. The Food and Drug Administration relies on analytical methods to quantify levels of use of cosmetic ingredients and support enforcement action against products that are not in compliance with the law. In this study, an efficient ultra high performance liquid chromatography with tandem mass spectrometry method was developed and validated for the determination of methylisothiazolinone and methylchloroisothiazolinone in selected cosmetic products. The lower limit of quantitation was determined to be 0.1 mug/g for both preservatives. A survey of 24 cosmetic products was conducted and found concentrations of methylisothiazolinone and methylchloroisothiazolinone ranging from not quantified, or below the lower limit of quantitation, to 89.64 mug/g and not quantified to 10.31 mug/g, respectively.

Category: Journal Article
PubMed ID: #26103935 DOI: 10.1002/jssc.201500365
Includes FDA Authors from Scientific Area(s): Food
Entry Created: 2016-02-19