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J Microbiol Methods 2016 Dec;131:7-9

A simplified mycobacterial growth inhibition assay (MGIA) using direct infection of mouse splenocytes and the MGIT system.

Yang AL, Schmidt TE, Stibitz S, Derrick SC, Morris SL, Parra M


We describe a simplified Mycobacterial Growth Inhibition Assay (MGIA) for pre-clinical assessment of vaccine-mediated protection in mice. The assay is accomplished by directly infecting splenocytes from vaccinated mice with Mycobacterium tuberculosis and quantifying mycobacteria using Mycobacterial Growth Indicator Tubes (MGIT). Vaccine-mediated immunogenicity detected by this assay correlated with protection.

Category: Journal Article
PubMed ID: #27650198 DOI: 10.1016/j.mimet.2016.09.010
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2016-08-29 Entry Last Modified: 2017-06-18