Scientific Publications by FDA Staff

Cell Rep 2018 Oct 9;25(2):312-20

Entry, replication, immune evasion, and neurotoxicity of synthetically engineered bat-borne mumps virus.

Kruger N, Sauder C, Huttl S, Papies J, Voigt K, Herrler G, Hardes K, Steinmetzer T, Orvell C, Drexler JF, Drosten C, Rubin S, Muller MA, Hoffmann M

Bats harbor a plethora of viruses with an unknown zoonotic potential. In-depth functional characterization of such viruses is often hampered by a lack of virus isolates. The genome of a virus closely related to human mumps viruses (hMuV) was detected in African fruit bats, batMuV. Efforts to characterize batMuV were based on directed expression of the batMuV glycoproteins or use of recombinant chimeric hMuVs harboring batMuV glycoprotein. Although these studies provided initial insights into the functionality of batMuV glycoproteins, the host range, replication competence, immunomodulatory functions, virulence, and zoonotic potential of batMuV remained elusive. Here, we report the successful rescue of recombinant batMuV. BatMuV infects human cells, is largely resistant to the host interferon response, blocks interferon induction and TNF-alpha activation, and is neurotoxic in rats. Anti-hMuV antibodies efficiently neutralize batMuV. The striking similarities between hMuV and batMuV point at the putative zoonotic potential of batMuV.