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EBioMedicine 2019 May;43:307-16

Development and validation of plasma miRNA biomarker signature panel for the detection of early HIV-1 infection.

Biswas S, Haleyurgirisetty M, Lee S, Hewlett I, Devadas K


BACKGROUND: Accurate laboratory diagnosis of HIV is essential to reduce the risk of HIV-positive individuals transmitting HIV-1 infection. The goal of this study was to identify and assess a panel of host derived plasma miRNAs that could to serve as a prognostic and predictive biomarker to detect early/acute HIV-1 infection. METHODS: A total of 372 microRNAs were analyzed in nine plasma samples from HIV-1 infected individuals in the early phase of infection and three healthy controls using the miRNA PCR-array. Seventeen microRNAs were selected and validated in 80 plasma samples from HIV-1 infected individuals in the early phase of infection (20 each of eclipse stage, RNA+ stage, Ag+stage, and Ag+Ab+ stage of HIV-1 patients) and 25 healthy controls. Using the validation study results a plasma miRNA panel was developed and evaluated to detect early/acute HIV-1 infection in 49 blinded samples. FINDING: We identified an miRNA panel (PeHIV-1) containing four differentially expressed miRNAs (miR-16-5p, miR-20b-5p, miR-195-5p, and miR-223-3p) that could distinguish early HIV-1 infection from healthy controls with high AUC (1.000[1.00-1.00]), sensitivity (100%), and specificity (100%).We also found that miR-223-3p demonstrates 100% sensitivity and specificity (AUC 1.00[1.00-1.00]) and could distinguish eclipse stage of HIV-1 infection from healthy controls. To detect eclipse stage of HIV-1 infection we also developed a four-miRNA based (miR-16-5p, miR-206, let-7g-3p, and miR-181c-3p) panel (PE) with AUC 0.999 (0.995-1.000), 100% sensitivity and 95.8% specificity. INTERPRETATION: The miRNA panel, PeHIV-1 is a potential biomarker for detecting early/acute stage of HIV-1infection and could help initiate early antiretroviral treatment, thus preventing the spread of HIV-1 infection.

Category: Journal Article
PubMed ID: #31005516 DOI: 10.1016/j.ebiom.2019.04.023
PubMed Central ID: #PMC6557912
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2018-11-04 Entry Last Modified: 2019-06-23