A customer from the united states reported to biomérieux a misidentification of an american proficiency institute (api) survey sample in association with the vitek® 2 anc test kit.An internal biomérieux investigation was performed.The organism was subbed on cba media and grown anaerobically for 48 hrs.Testing included two (2) customer lots and a random lot of anc cards.Vitek® ms and 16s sequencing was also performed.On all cards tested, a very good (95%) or excellent id (98%) of b.Thetaiotamicron was obtained.For testing on the vitek® ms, a 99.9% identity match of b.Thetaiotamicron was obtained.16s sequencing gave a 100% identity match to b.Thetaiotamicron.When the organism's 16s sequence was compared against the 16s sequence for b.Uniformis, there was only a 92% identity match.Therefore, the final identification is b.Thetaiotamicron.Vitek® 2 anc cards were determined to be performing as expected and no further action is necessary.
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