An investigation was performed.The cap d-10 survey specimen was not graded due to the lack of participant and referee consensus.Referees demonstrated 49.2 % and participants 56.2 % consensus for correct identification of all organisms.The bmx internal cap d-10 strain was rehydrated and subcultured under anaerobic conditions.Testing included individual organism suspensions on anc cards from four (4) different lots, in duplicate.Testing was also performed with vitek® ms.The eight (8) anc cards tested resulted in low discrimination identifications of multiple clostridium species, not the expected result.The vitek® ms resulted in the expected identification of propionibacterium granulosum (99.5%).A review of customer's results against expected reactions for p.Granulosum, demonstrated several atypical negative reactions (ellm, pyra, arg, pvate, amani, drib).Many survey participants additionally entered atypical positive morphology (cocci) according to the anc knowledge base all contributing to the misidentification.An increased number of atypical negative results can indicate a strain with decreased viability, user set up error or an atypical strain.The investigation concluded the submitted isolate has an atypical biochemical profile.
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