A customer from the united states contacted biomérieux to report the occurrence of a misidentification of eggerthella lenta ((b)(6)) as actinomyces odontolyticus in association with the vitek® 2 anaerobic and corynebacteria (anc) id test kit.The customer reported the result to (b)(6) as actinomyces species.Upon receiving the (b)(6) report indicating eggerthella lenta, repeat testing was performed and obtained eggerthia catenaformis.There is no indication or report from the laboratory that the discrepant result led to any adverse event related to any patient's state of health.There was no patient directly associated with the (b)(6) strain.Culture submittal was requested by biomérieux to be available for internal investigation; however, the customer stated it was likely no longer viable.Biomérieux investigation will be initiated.
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An internal biomérieux investigation was performed with results as follows: the biomérieux internal cap d-17 swab was re-hydrated and subcultured to remel anaerobic blood agar.Testing included individual organism suspensions with anc cards from two different card lots as well as the vitek® ms instrument.The anc cards resulted in low discrimination identifications of e.Lenta/clostridium group.The vitek® ms instrument identified the isolate as e.Lenta with a 99.9% confidence value.Vitek® 2 anc cards are performing as expected.A review of the customer's actinomyces odontolyticus reactions demonstrated five (5) atypical positive reactions (leua, phea, tyra, appa, phos) and one (1) atypical negative reaction (arg) for an identification of e.Lenta according to the anc knowledge base.A review of the customer's e.Catenaformis reactions only demonstrated one atypical reaction as the offline testing indicated the isolate to be aerobic instead of anaerobic for an identification of e.Lenta according to the anc knowledge base.An increased number of atypical reactions can indicate contamination, mixed culture, compromised viability of the strain, use of non- recommended media or other user set up errors or an atypical strain.
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