A customer from france reported to biomérieux a misidentification of proponobacterium acnes in association with the vitek® 2 anc test kit.(b)(4).The customer submitted lab reports for evaluation.An investigation was performed.The customer set up the anc card from a 24 hour culture grown on columbia sheep blood agar under anaerobic conditions.Two anc lab reports were submitted showing an excellent identification of atobopium vaginae.Both lab reports showed two atypical negative reactions (ellm, arg) for an identification of proponobacterium acnes according to the anc knowledge base.Three lab reports were submitted for the quality control strain, corynebacterium striatum atcc baa-1293, which showed passing qc results.A review of quality records confirmed anc lot 244097720 had no issues with qc performance testing and met final qc release criteria.In conclusion, an increased number of atypical negative results can indicate a strain with decreased viability, user set up error or an atypical strain.Without the strain or raw data it's not possible to further evaluate the cause of the misidentification.
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